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Rad51 Rabbit mAb #58574

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产品详情

产品名称Rad51 Rabbit mAb

来源种属Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB;IHC;ICC/IF

种属反应性Human Mouse Rat

特异性Rad51 Antibody detects endogenous levels of total Rad51

标记Unconjugated

别名RAD51 homolog A; DNA repair protein RAD51 homolog 1; RAD51A; RECAhomolog S. cerevisiae; RAD51A; RECA; Rad 51; RecA homolog E. coli; RecA like protein;

数据库入口号Q06609

Uniprot Q06609

计算分子量37 kDa

实际分子量37 kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

应用详情
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Rad51 Rabbit mAb at 1/1k dilution
Lane 1 : 293 whole cell lysates
Lane 2 : K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 6 seconds
All lanes: Rad51 Rabbit mAb at 1/1k dilution
Lane 1 : RAW264.7 whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 3 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 seconds
All lanes:Rad51 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Rad51 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Rad51 using 58574 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Rad51 antibody (58574)
ICC/IF staining of Rad51 in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 58574 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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