有货
All lanes: Raf1 Rabbit mAb at 1/1k dilutionLane 1 : MCF-7 whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : HepG2 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 73 kDa Observed band size: 73 kDaExposure time: 4 seconds
All lanes: Raf1 Rabbit mAb at 1/1k dilutionLane 1 : Mouse brain lysates Lane 2 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 73 kDa Observed band size: 73 kDaExposure time: 5 seconds
All lanes :Raf1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type A549 cell lysateLane 2 : Raf1 knockdown A549 cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence Raf1 antibody (58660) ICC/IF staining of Raf1 in MCF7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58660 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
