有货
All lanes: Hsp60 Rabbit mAb at 1/1k dilutionLane 1 : HeLa whole cell lysates Lane 2 : 293T whole cell lysates Lane 3 : 3T3 whole cell lysates Lane 4 : Mouse heart lysates Lane 5 : PC-12 whole cell lysates Lane 6 : Rat heart lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 61 kDa Observed band size: 61 kDaExposure time: 5 seconds
All lanes:Hsp60 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : Hsp60 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Hsp60 using 58772 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Hsp60 antibody (58772) ICC/IF staining of Hsp60 in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58772 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control. Nuclei were counterstained with DAPI.
