有货
All lanes: Hsc70 Rabbit mAb at 1/1k dilutionLane 1 : HeLa whole cell lysates Lane 2 : C6 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 71 kDa Observed band size: 71 kDaExposure time: 4 seconds
All lanes: Hsc70 Rabbit mAb at 1/1k dilutionLane 1 : Mouse brain lysates Lane 2 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 71 kDa Observed band size: 71 kDaExposure time: 3 seconds
All lanes:Hsc70 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : Hsc70 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Hsc70 using 58784 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Hsc70 antibody (58784) ICC/IF staining of Hsc70 in MCF7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58784 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
