有货
All lanes: MLKL Rabbit mAb at 1/1k dilutionLane 1 : HUVEC whole cell lysates Lane 2 : A375 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 54 kDa Observed band size: 54 kDaExposure time: 7 seconds
All lanes:MLKL Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : MLKL knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon tissue stained for MLKL using 58819 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence MLKL antibody (58819) ICC/IF staining of MLKL in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58819 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
