有货
All lanes: Smad2 Rabbit mAb at 1/1k dilutionLane 1 : JK whole cell lysates Lane 2 : K562 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 52 kDa Observed band size: 58 kDaExposure time: 11 seconds
All lanes:Smad2 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HT-1080 cell lysateLane 2 : Smad2 knockdown HT-1080 cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human stomach carcinoma tissue stained for Smad2 using 58887 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Smad2 antibody (58887) ICC/IF staining of Smad2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58887 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
