有货
All lanes: Caspase-3 p12 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 12,29,32 kDa
Exposure time: 7 seconds
All lanes: Caspase-3 p12 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse brain lysates Lane 2 : Mouse skin lysates Lane 3 : Rat brain lysates Lane 4 : Rat skin lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 4 seconds
All lanes :Caspase-3 p12 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Caspase-3 p12 knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Caspase-3 p12 using 59043 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Caspase-3 p12 antibody (59043) ICC/IF staining of Caspase-3 p12 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59043 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
