有货
All lanes: HDAC4 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : K562 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 119 kDa Observed band size: 140 kDaExposure time: 5 seconds
All lanes:HDAC4 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HCT116 cell lysateLane 2 :HDAC4 Rabbit mAb knockdown HCT116 cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence HDAC4 antibody (59145) ICC/IF staining of HDAC4 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59145 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
