有货
All lanes: Src Rabbit mAb at 1/1k dilutionLane 1 : 293 whole cell lysates Lane 2 : A375 whole cell lysates Lane 3 : C6 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 60 kDa Observed band size: 60 kDaExposure time: 7 seconds
All lanes: Src Rabbit mAb at 1/1k dilutionLane 1 : Mouse spleen lysates Lane 2 : Mouse lung lysates Lane 3 : Rat spleen lysates Lane 4 : Rat heart lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 60 kDa Observed band size: 60 kDaExposure time: 6 seconds
All lanes:Src Rabbit mAb at 1/1k dilutionLane 1 : Wild-type A549 cell lysateLane 2 :Src Rabbit mAb knockdown A549 cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Src using 59230 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Src antibody (59230) ICC/IF staining of Src in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59230 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
