有货
All lanes: ERCC1 Rabbit mAb at 1/1k dilutionLane 1 : HT-1080 whole cell lysates Lane 2 : PC-3 whole cell lysates Lane 3 : SK-OV-3 whole cell lysates Lane 4 : HCT116 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 33 kDa Observed band size: 36 kDaExposure time: 4 seconds
All lanes:ERCC1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 :ERCC1 Rabbit mAb knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded mouse colon tissue stained for ERCC1 using 59285 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence ERCC1 antibody (59285) ICC/IF staining of ERCC1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59285 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
