yes
All lanes : CSPG4 Rabbit mAb at 1/1k dilutionLane 1 : A172 whole cell lysates Lane 2 : A375 whole cell lysates Lane 3 : Hela whole cell lysatesLysates/proteins at 20 µg per lane.Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 251 kDa Observed band size: 251 kDaExposure time: 20 seconds
All lanes : CSPG4 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysate Lane 2 : CSPG4 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon carcinom tissue stained for CSPG4 using 59290 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CSPG4 antibody (59290) 59290 staining CSPG4 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59290 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 59290 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
