有货
All lanes: NUMB Rabbit mAb at 1/1k dilutionLane 1 : Ramos whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 71 kDa Observed band size: 71 kDaExposure time: 6 seconds
All lanes:NUMB Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HT-1080 cell lysateLane 2 :NUMB Rabbit mAb knockdown HT-1080 cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for NUMB using 59301 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence NUMB antibody (59301) ICC/IF staining of NUMB in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59301 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
