有货
All lanes: Annexin V Rabbit mAb at 1/1k dilutionLane 1 : 293 whole cell lysates Lane 2 : MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 36 kDa Observed band size: 33 kDaExposure time: 7 seconds
All lanes: Annexin V Rabbit mAb at 1/1k dilutionLane 1 : Rat heart lysates Lane 2 : Rat kidney lysates Lane 3 : Mouse spleen lysates Lane 4 : Mouse lung lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 36 kDa Observed band size: 33 kDaExposure time: 4 seconds
All lanes:Annexin V Rabbit mAb at 1/1k dilutionLane 1 : Wild-type A549 cell lysateLane 2 :Annexin V Rabbit mAb knockdown A549 cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence Annexin V antibody (59326) ICC/IF staining of Annexin V in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59326 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
