有货
All lanes: AAMP Rabbit mAb at 1/1k dilutionLane 1 : A375 whole cell lysates Lane 2 : MCF-7 whole cell lysates Lane 3 : Mouse brain lysates Lane 4 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 47 kDa Observed band size: 52 kDaExposure time: 6 seconds
All lanes:AAMP Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 :AAMP Rabbit mAb knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence AAMP antibody (60181) ICC/IF staining of AAMP in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 60181 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control. Nuclei were counterstained with DAPI.
