产品详情
产品名称Histone H3(Acetyl-Lys27) Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Affinity purification using immunogen.
应用WB;IHC;IF;IP;ELISA
种属反应性Human;Mouse;Rat;Monkey;Arabidopsis thaliana
特异性The Histone H3(Acetyl Lys27) Rabbit Polyclonal Antibody detects endogenous Histone H3 (Acetyl Lys27) protein.
免疫原类型peptide
免疫原描述A synthetic acetylated peptide corresponding to residues surrounding Lys27 of human histone H3.
基因/蛋白名称Histone H3(Acetyl-Lys27)
标记Unconjugated
别名H3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody
数据库入口号Swiss-Prot#:P68431
Uniprot
P68431
实际分子量17kd
浓度1.0mg/ml
配方PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
保存Store at -20˚C
应用详情
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; IP 1:200; ELISA 1:5000
Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using #HW077 diluted at 1:1,000.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys27) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Hela cell. Histone H3 (Acetyl Lys27) Polyclonal Antibody(red) was diluted at 1:200(4° overnight).
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
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