产品详情
产品名称Flag-Tag Mouse Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用WB IF IP
种属反应性Human;Mouse;Rat
特异性The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.
基因/蛋白名称Flag-Tag
标记Unconjugated
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:10000-50000; IP 1:50-1:200; IF 1:1000 -3000
1ug Flag fusion protein+ Primary antibody #T519 dilution at 1:10,000.
IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb #T519 at a 1:2000 dilution (blue DAPI, red anti-Flag).
IP antibody use£º5ug Flag Mouse IgG1 per ml Lysate£¬WB 1:5000
1¡¢untransfected 293 cell lysate
2¡¢transfected 293 cell lysate with Flag-tag fusion protein
3¡¢IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4¡¢IP (transfected 293+anti- Flag mAb+ Protein G agarose)
5¡¢IP (transfected 293+Protein G)
6¡¢Recombinant protein (E.coli).
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
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