产品详情
产品名称Flag-Tag Mouse Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化Protein A purified
应用WB;ELISA;IP;IF;CoIP
种属反应性Species independent
特异性This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
基因/蛋白名称Flag-Tag
标记Unconjugated
别名Flag tag;Flag-tag;DDDDK TAG;DDDDK-TAG;DYKDDDDK tag;DYKDDDDK-tag
配方PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
保存Store at -20℃/1 year
应用详情
WB 1:10000-1:50000;
ELISA 1:20000-1:50000;
IP 1:50-1:200;
IF 1:1000-1:3000
Western Blot analysis of 293F cells transfected or non-tansfected Flag-Tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected Flag-Tag expression vector. Secondary antibody was diluted at 1:10000
Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody was diluted at 1:10000
1) Input: 293T transfected N-flag protein Lysate 2) IP product: IP dilute 1: 200, 293T transfected N-flag protein Lysate Western blot analysis: primary antibody : 1:10000 Secondary antibody: Goat anti-Mouse IgG, 1: 5000
Immunofluorescence analysis of 293T transfected FLAG and GFP tag protein. 1, FLAG primary Antibody was diluted at 1:1000(4℃ overnight), GFP primary Antibody was diluted at 1:1000(4℃ overnight)
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
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Yes
