Yes
All lanes : FOXO3A (Phospho-Ser253) Rabbit mAb at 1/1k dilutionLane 1 : K562 whole cell lysatesLane 2 : C6 whole cell lysatesLane 3 : 3T3 whole cell lysatesLane 4 : 3T3 treated with Lambda Protein Phosphatase for 30min whole cellLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 71 kDa Observed band size: 90 kDaExposure time: 6 seconds
All lanes : FOXO3a(Phospho-S253) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 3 : MCF7 whole cell lysates
Lane 4 : Mouse liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa
Exposure time: 12 seconds
Formalin-fixed;paraffin-embedded human pancreas tissue stained for FOXO3a(Phospho-S253) using 13372 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded mouse pancreas tissue stained for FOXO3a(Phospho-S253) using 13372 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded rat pancreas tissue stained for FOXO3a(Phospho-S253) using 13372 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence FOXO3a(Phospho-S253) antibody (13372)
ICC/IF staining of FOXO3a(Phospho-S253) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13372 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
