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位置: 首页 > KO/KD抗体 > Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb

Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb#14158

是否有货: Yes
产品详情

产品名称Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB IHC ICC/IF

种属反应性Human;Mouse

特异性Phospho-Glycogen synthase 1 (S641) Antibody detects endogenous levels of total Phospho-Glycogen synthase 1 (S641)

免疫原描述A synthesized peptide derived from human Phospho-Glycogen synthase 1 (S641)

标记Unconjugated

别名glycogen [starch] synthase, muscle; glycogen synthase, muscle; GYS;

数据库入口号Uniprot:P13807

Uniprot P13807

计算分子量84 kDa

实际分子量85 kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

应用详情
WB:1:1000~1:2000
IHC:1:50~1:200
ICC/IF:1:50~1:200
All lanes : Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : 3T3 treated with Lambda Protein Phosphatase for 30min whole cell
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with Lambda Protein Phosphatase for 30min whole cell
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 85 kDa
Exposure time: 5 seconds
All lanes : Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Glycogen synthase 1 (Phospho-Ser641) knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon tissue stained for Glycogen synthase 1 (Phospho-Ser641) using 14158 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Glycogen synthase 1 (Phospho-Ser641) antibody (14158)
ICC/IF staining of Glycogen synthase 1 (Phospho-Ser641) in A549 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14158 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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