Yes
All lanes: Src (Phospho-Tyr419) Rabbit mAb at 1/1k dilution
Lane 1 : A431 whole cell lysates
Lane 2 : A431 treated with pervanadate whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 7 seconds
All lanes: Src (Phospho-Tyr419) Rabbit mAb at 1/1k dilution
Lane 1 : Rat bladder lysates
Lane 2 : Mouse bladder lysates Lane 3 : Mouse testis lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 6 seconds
All lanes :Src (Phospho-Tyr419) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 :Src (Phospho-Tyr419) knockdown A549 cell lysate
Lysates/proteins at 20 µg per lane.
