Yes
All lanes : Phospho-p90 S6K alpha 1 (S380) Rabbit mAb at 1/1k dilution Lane 1 : A549 whole cell lysates Lane 2 : A431 whole cell lysates Lane 3 : JK whole cell lysates Lane 4 : 3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 5 seconds
All lanes : Phospho-p90 S6K alpha 1 (S380) Rabbit mAb at 1/1k dilution Lane 1 : Mouse Lung lysates Lane 2 : Rat Skin lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 5 seconds
All lanes : Phospho-p90 S6K alpha 1 (S380) Rabbit mAb at 1/1k dilution Lane 1 : Hela whole cell lysates Lane 2 : Hela treated with 200nM PMA for 30min whole cell lysates Lane 3 :Hela treated with 100ng/ml EGF for 20min whole cell lysates Lane 4 : JK whole cell lysates Lane 5 : JK treated with 200nM PMA for 30min whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 6 seconds
Immunocytochemistry/ Immunofluorescence p-p90 S6K alpha 1 (S380) antibody (14173) 14173 staining p-p90 S6K alpha 1 (S380) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14173 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 14173 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
All lanes : Phospho-p90 S6K alpha 1 (S380) Rabbit mAb at 1/1k dilution Lane 1 : Wild-type Hela cell lysate Lane 2 : Phospho-p90 S6K alpha 1 (S380) knockdown Hela cell lysate Lysates/proteins at 20 µg per lane.
