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位置: 首页 > KO/KD抗体 > Raf1 (Phospho-Ser621) Rabbit mAb

Raf1 (Phospho-Ser621) Rabbit mAb#14227

是否有货: Yes
产品详情

产品名称Raf1 (Phospho-Ser621) Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB

种属反应性Human;Mouse;Rat

特异性Phospho-Raf1 (S621) Antibody detects endogenous levels of total Phospho-Raf1 (S621)

免疫原描述A synthesized peptide derived from human Phospho-Raf1 (S621)

标记Unconjugated

别名C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;

数据库入口号Uniprot:P04049

Uniprot P04049

计算分子量73kDa

实际分子量73kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

应用详情

WB:1:500~1:2000

All lanes: Raf1 (Phospho-Ser621) Rabbit mAb at 1/1k dilution
Lane 1 : Hela treated with alkaline phosphatase whole cell lysates
Lane 2 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 7 seconds
All lanes: Raf1 (Phospho-Ser621) Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 10 seconds
All lanes: Raf1 (Phospho-Ser621) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Raf1 (Phospho-Ser621) knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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Email Address

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