产品详情
产品名称COMP Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total COMP protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant fusion protein of human COMP (NP_000086.2).
基因/蛋白名称COMP
标记Unconjugated
别名COMP;EDM1;EPD1;MED;PSACH;THBS5;TSP5
数据库入口号Uniprot:P49747
GeneID:1311
Uniprot
P49747
Gene ID
1311
实际分子量110KDa
配方PBS containing 50% glycerol, preserved with proclin300 or sodium azide ,pH 7.3
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:500-1:5000;
IF 1:50-1:200
Western blot analysis of various lysates using COMP Antibody at 1:1000 dilution;
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution;
Lysates/proteins: 25 ug per lane;
Blocking buffer: 3% nonfat dry milk in TBST;
Exposure time: 1s.
Western blot analysis of various lysates using COMP Antibody at 1:1000 dilution;
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution;
Lysates/proteins: 25 ug per lane;
Blocking buffer: 3% nonfat dry milk in TBST;
Exposure time: 30s.
Immunofluorescence analysis of paraffinembedded Rat cartilage using COMP Antibody at dilution of 1:200 (40x lens);
Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution;
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffinembedded Mouse cartilage using COMP Antibody at dilution of 1:200 (40x lens);
Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution;
Blue: DAPI for nuclear staining.
The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED).
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et al,The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease. In Bone Joint Res on 2020 Sep 20 by Mei Ma, Xiao Liang, et al..PMID:33005397
, (2020),
PMID:
33005397
et al,Electroacupuncture improves motor function of rats with osteoarthritis by alleviating joint inflammation through the Wnt-7B/β-catenin signaling pathwayInNan Fang Yi Ke Da Xue Xue BaoOn2023 Apr 20byX Zheng?1,?S Gao et al..PMID: 37202195
, (2023),
PMID:
37202195