免费电话:400-9961-933
邮箱:info@sabbiotech.com
欢迎光临本站
川博智能体 English
位置: 首页 > 多克隆抗体 > COMP Antibody

COMP Antibody#33060

是否有货: 有货
产品详情

产品名称COMP Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化Affinity purification

应用WB,IF

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of total COMP protein.

免疫原类型Recombinant Protein

免疫原描述Recombinant fusion protein of human COMP (NP_000086.2).

基因/蛋白名称COMP

标记Unconjugated

别名COMP;EDM1;EPD1;MED;PSACH;THBS5;TSP5

数据库入口号Uniprot:P49747
GeneID:1311

Uniprot P49747

Gene ID 1311

实际分子量110KDa

配方PBS containing 50% glycerol, preserved with proclin300 or sodium azide ,pH 7.3

保存Store at -20˚C. Avoid freeze / thaw cycles.

应用详情
WB 1:500-1:5000;
IF 1:50-1:200
Western blot analysis of various lysates using COMP Antibody at 1:1000 dilution;
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution;
Lysates/proteins: 25 ug per lane;
Blocking buffer: 3% nonfat dry milk in TBST;
Exposure time: 1s.
Western blot analysis of various lysates using COMP Antibody at 1:1000 dilution;
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution;
Lysates/proteins: 25 ug per lane;
Blocking buffer: 3% nonfat dry milk in TBST;
Exposure time: 30s.
Immunofluorescence analysis of paraffinembedded Rat cartilage using COMP Antibody at dilution of 1:200 (40x lens);
Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution;
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffinembedded Mouse cartilage using COMP Antibody at dilution of 1:200 (40x lens);
Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution;
Blue: DAPI for nuclear staining.
The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED).

如果您使用该产品33060发表了文章,请通知我们,让我们可以引用您的文献。

et al,The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease. In Bone Joint Res on 2020 Sep 20 by Mei Ma, Xiao Liang, et al..PMID:33005397 , (2020),
PMID: 33005397
et al,Electroacupuncture improves motor function of rats with osteoarthritis by alleviating joint inflammation through the Wnt-7B/β-catenin signaling pathwayInNan Fang Yi Ke Da Xue Xue BaoOn2023 Apr 20byX Zheng?1,?S Gao et al..PMID: 37202195 , (2023),
PMID: 37202195

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

info@sabbiotech.com