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sfGFP Antibody#48545

是否有货: Yes
产品详情

产品名称sfGFP Antibody

纯化Peptide affinity purified.

应用WB

免疫原描述Synthetic peptide RDHMVLHEYVNAAGITC linked to KLH.

标记Unconjugated

配方1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

应用详情

WB: 1:1,000

 

Western blot analysis of GFP11-tag on fusion protein using anti-GFP11-tag antibody at 1/1,000 dilution.

Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.

如果您使用该产品48545发表了文章,请通知我们,让我们可以引用您的文献。

et al,Enhancing mitochondrial proteolysis alleviates alpha-synuclein-mediated cellular toxicity. In NPJ Parkinsons Dis on 2024 Jun 21 by Xi Zhang, Linhao Ruan,et al..PMID:38906862 , (2024),
PMID: 38906862

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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