产品详情
产品名称Integrin beta 1 Rabbit mAb
克隆号SA40-08
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB IHC ICC/IF
种属反应性Human;Mouse;Rat;Zebrafish
免疫原描述recombinant protein
标记Unconjugated
别名beta1 integrin antibody
CD29 antibody
Fibronectin receptor subunit beta antibody
FNRB antibody
Glycoprotein IIa antibody
GP IIa antibody
GPIIA antibody
Integrin beta-1 antibody
integrin VLA-4 beta subunit antibody
Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
ITB1_HUMAN antibody
ITGB1 antibody
MDF2 antibody
MSK12 antibody
OTTHUMP00000019420 antibody
Very late activation protein, beta polypeptide antibody
VLA BETA antibody
VLA-4 subunit beta antibody
VLA-BETA antibody
VLAB antibody
VLAbeta antibody
数据库入口号Swiss-Prot#:P05556
Uniprot
P05556
计算分子量88 kDa
实际分子量140 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Integrin beta 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Integrin beta 1 antibody. Counter stained with hematoxylin.
All lanes: Integrin beta 1 Rabbit mAb at 1/1k dilutionLane 1 : HeLa whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : HepG2 whole cell lysates Lane 4 : MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 88 kDa Observed band size: 140 kDaExposure time: 9 seconds
All lanes: Integrin beta 1 Rabbit mAb at 1/1k dilutionLane 1 : Mouse skin lysates Lane 2 : Rat skin lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 88 kDa Observed band size: 140 kDaExposure time: 7 seconds
All lanes :Integrin beta 1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HAP1 cell lysateLane 2 : Integrin beta 1 knockdown HAP1 cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence Integrin beta 1 antibody (48603) ICC/IF staining of Integrin beta 1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 48603 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
Integrins are transmembrane receptors that mediate the attachment between a cell and its surroundings, such as other cells or the extracellular matrix (ECM). Integrins are obligate heterodimers containing two distinct chains, called the α (alpha) and β (beta) subunits. The molecular mass of the integrin subunits can vary from 90 kDa to 160 kDa. Beta subunits have four cysteine-rich repeated sequences. Both α and β subunits bind several divalent cations. Integrins have two main functions: Attachment of the cell to the ECM and signal transduction from the ECM to the cell. However, they are also involved in a wide range of other biological activities, including immune patrolling, cell migration, and binding to cells by certain viruses, such as adenovirus, echovirus, hantavirus, and foot and mouth disease viruses. Research studies have implicated β1 integrin in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis.
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