免费电话:400-9961-933
邮箱:info@sabbiotech.com
欢迎光临本站
川博智能体 English
位置: 首页 > 重组兔单抗 > LAMP2 Rabbit mAb

LAMP2 Rabbit mAb #48611

是否有货: Yes
产品详情

产品名称LAMP2 Rabbit mAb

克隆号SA46-01

来源种属Recombinant Rabbit

克隆性Monoclonal antibody

纯化ProA affinity purified

应用WB IHC ICC/IF

种属反应性Human;Mouse;Rat

免疫原描述recombinant protein

标记Unconjugated

别名CD107 antigen-like family member B antibody
CD107b antibody
LAMP 2 antibody
Lamp 2a antibody
LAMP-2 antibody
LAMP2 antibody
LAMP2_HUMAN antibody
Lysosome-associated membrane glycoprotein 2 antibody
Lysosome-associated membrane protein 2 antibody

数据库入口号Swiss-Prot#:P13473

Uniprot P13473

计算分子量45 kDa

实际分子量110 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200


Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
All lanes: LAMP2 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse liver lysates
Lane 2 : Rat liver lysates
Lane 3 : JAR whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 110 kDa
Exposure time: 4 seconds
All lanes :LAMP2 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : LAMP2 knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence LAMP2 antibody (48611)
ICC/IF staining of LAMP2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 48611 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107A) and LAMP-2 (also designated CD107B) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans Golgi network and are transported intracellularly via a pathway that is distinct from the clathrin-coated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders.

如果您使用该产品48611发表了文章,请通知我们,让我们可以引用您的文献。

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

info@sabbiotech.com