产品详情
产品名称LAMP2 Rabbit mAb
克隆号SA46-01
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB IHC ICC/IF
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名CD107 antigen-like family member B antibody CD107b antibody LAMP 2 antibody Lamp 2a antibody LAMP-2 antibody LAMP2 antibody LAMP2_HUMAN antibody Lysosome-associated membrane glycoprotein 2 antibody Lysosome-associated membrane protein 2 antibody
数据库入口号Swiss-Prot#:P13473
Uniprot
P13473
计算分子量45 kDa
实际分子量110 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.
All lanes: LAMP2 Rabbit mAb at 1/1k dilutionLane 1 : Mouse liver lysates Lane 2 : Rat liver lysates Lane 3 : JAR whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 45 kDa Observed band size: 110 kDaExposure time: 4 seconds
All lanes :LAMP2 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HAP1 cell lysateLane 2 : LAMP2 knockdown HAP1 cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence LAMP2 antibody (48611) ICC/IF staining of LAMP2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 48611 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107A) and LAMP-2 (also designated CD107B) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans Golgi network and are transported intracellularly via a pathway that is distinct from the clathrin-coated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders.
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