产品详情
产品名称PKC delta Rabbit mAb
克隆号JJ093-02
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名CVID9 antibody
D14Ertd420e antibody
Kinase PKC delta antibody
KPCD antibody
KPCD_HUMAN antibody
MAY 1 antibody
MAY1 antibody
MGC49908 antibody
nPKC delta antibody
nPKC-delta antibody
PCKd antibody
PKC d antibody
PKC delta antibody
PKCD antibody
PKCdelta antibody
PRKC D antibody
PRKC delta antibody
Prkcd antibody
Protein Kinase C delta antibody
Protein kinase C delta type antibody
Protein kinase C delta VIII antibody
Protein Kinase Cdelta antibody
Tyrosine protein kinase PRKCD antibody
数据库入口号Swiss-Prot#:Q05655
Uniprot
Q05655
计算分子量78 kDa
实际分子量78 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
ICC staining PKC delta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PKC delta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PKC delta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PKC delta in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: PKC delta Rabbit mAb at 1/1k dilutionLane 1 : HeLa whole cell lysates Lane 2 : Mouse brain lysates Lane 3 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 78 kDa Observed band size: 78 kDaExposure time: 6 seconds
All lanes:PKC delta Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 :PKC delta Rabbit mAb knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
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