Yes
All lanes: CHD3 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates Lane 2 : Mouse brain lysates Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 227 kDa
Observed band size: 260 kDa
Exposure time: 9 seconds
All lanes:CHD3 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : CHD3 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast tissue stained for CHD3 using 56204 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CHD3 antibody (56204) ICC/IF staining of CHD3 in C6 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56204 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
