Aspartate Aminotransferase Rabbit mAb#56284
评价
Yes
All lanes : Aspartate Aminotransferase Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : MCF7 whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : K562 whole cell lysates
Lane 5 : Raw264.7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 41 kDa
Exposure time: 10 seconds
All lanes : Aspartate Aminotransferase Rabbit mAb at 1/1k dilution
Lane 1 : Mouse Brain lysates
Lane 2 : Mouse Kidney lysates
Lane 3 : Rat Brain lysates
Lane 4 : Rat Kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 41 kDa
Exposure time: 5 seconds
All lanes : Aspartate Aminotransferase Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Aspartate Aminotransferase knockdown HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Aspartate Aminotransferase using 56284 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for Aspartate Aminotransferase using 56284 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Aspartate Aminotransferase antibody (56284)
56284 staining Aspartate Aminotransferase in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56284 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control, purified 56284 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500.
Nuclei were counterstained with DAPI.
All lanes :Aspartate Aminotransferase Rabbit mAb at 1/1k dilution Lane 1 : Wild-type HAP1 cell lysate Lane 2 : Aspartate Aminotransferase knockdown HAP1 cell lysate Lysates/proteins at 20 µg per lane.
