Yes
All lanes : BMP6 Rabbit mAb at 1/1k dilution Lane 1 : HUVEC whole cell lysates Lane 2 : 293T whole cell lysates Lane 3 : A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 42 kDa Exposure time: 3 seconds
All lanes : BMP6 Rabbit mAb at 1/1k dilution Lane 1 : Mouse Lung lysates Lane 2 : Rat Brain lysates Lane 2 : Rat Kidney lysates Lane 3 : Rat Lung lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 42 kDa Exposure time: 3 seconds
All lanes : BMP6 Rabbit mAb at 1/1k dilution Lane 1 : Wild-type HeLa cell lysate Lane 2 : BMP6 knockdown HeLa cell lysate Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for BMP6 using 56493 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat brain tissue stained for BMP6 using 56493 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence BMP6 antibody (56493) 56493 staining BMP6 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56493 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 56493 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
