有货
All lanes: MKLP1 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : A549 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 110 kDa Observed band size: 98 kDaExposure time: 7 seconds
All lanes:MKLP1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : MKLP1 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence MKLP1 antibody (58517) ICC/IF staining of MKLP1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58517 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
