有货
All lanes: AMPK alpha 1 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : HepG2 whole cell lysates Lane 4 : MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 64 kDa Observed band size: 64 kDaExposure time: 2 seconds
All lanes: AMPK alpha 1 Rabbit mAb at 1/1k dilutionLane 1 : Mouse kidney lysates Lane 2 : Mouse lung lysates Lane 3 : Rat kidney lysates Lane 4 : Rat lung lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 64 kDa Observed band size: 64 kDaExposure time: 6 seconds
All lanes:AMPK alpha 1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : AMPK alpha 1 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for AMPK alpha 1 using 58724 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence AMPK alpha 1 antibody (58724) ICC/IF staining of AMPK alpha 1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58724 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
