有货
All lanes: IKK alpha Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : 293 whole cell lysates Lane 3 : A375 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 85 kDa Observed band size: 85 kDaExposure time: 6 seconds
All lanes: IKK alpha Rabbit mAb at 1/1k dilutionLane 1 : Mouse lung lysates Lane 2 : Mouse brain lysates Lane 3 : Rat lung lysates Lane 4 : Rat heart lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 85 kDa Observed band size: 85 kDaExposure time: 8 seconds
All lanes:IKK Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : IKK knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for IKK alpha using 59000 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for IKK alpha using 59000 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence IKK alpha antibody (59000) ICC/IF staining of IKK alpha in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59000 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
