有货
All lanes: 14-3-3 gamma Rabbit mAb at 1/1k dilutionLane 1 : A549 whole cell lysates Lane 2 : HUVEC whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 28 kDa Observed band size: 28 kDaExposure time: 3 seconds
All lanes: 14-3-3 gamma Rabbit mAb at 1/1k dilutionLane 1 : Mouse skeletal muscle lysates Lane 2 : Rat skeletal muscle lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 28 kDa Observed band size: 28 kDaExposure time: 6 seconds
All lanes:14-3-3 gamma Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HAP1 cell lysateLane 2 :14-3-3 gamma Rabbit mAb knockdown HAP1 cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for 14-3-3 gamma using 59100 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence 14-3-3 gamma antibody (59100) ICC/IF staining of 14-3-3 gamma in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59100 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
