产品详情
产品名称Histone H3K79me2 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
免疫原类型Peptide
免疫原描述A synthetic peptide of human DiMethyl-Histone H3-K79
基因/蛋白名称Histone H3
标记Unconjugated
别名H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
数据库入口号Uniprot:Q16695
GeneID:8290
Uniprot
Q16695
Gene ID
8290
实际分子量17kDa
浓度1.0mg/ml
配方PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Western blot analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 antibody.
Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody.
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody.
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody.
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
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